Actinomycin D binds with highest affinity to nonribosomal DNA.
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چکیده
منابع مشابه
Structure-affinity relationships for the binding of actinomycin D to DNA
Molecular models of the complexes between actinomycin D and 14 different DNA hexamers were built based on the X-ray crystal structure of the actinomycin-d(GAAGCTTC)2 complex. The DNA sequences included the canonical GpC binding step flanked by different base pairs, nonclassical binding sites such as GpG and GpT, and sites containing 2,6-diamino-purine. A good correlation was found between the i...
متن کاملThe Radioautographic Detection of Dna with Tritiated Actinomycin D
The specificity with which actinomycin D is bound to DNA both in vivo and in test tube experiments (for a review, see Reich and Goldberg, 1964) and the recent availability of the drug in tritiated form with high specific activity have suggested its possible usefulness as a means to detect DNA radioautographically in fixed cytological preparations. Brachet and Ficq (1965) had previously employed...
متن کاملExperimental studies with actinomycin D.
Actinomycin D has been tested on mouse tu mors, with varying degrees of success, as an anti-neoplastic agent. Dramatic inhibition of some ascites (mouse) tumors has been reported by Sugiura (14) and Gregory et al. (6). Reilly (11) and Sugiura (14) are in agreement that actino-mycin D has slight antineoplastic activity against S-180, and Farber (3) demonstrated antitumor activity against the mal...
متن کاملSingle-strand DNA binding of actinomycin D with a chromophore 2-amino to 2-hydroxyl substitution.
A modified actinomycin D was prepared with a hydroxyl group that replaced the amino group at the chromophore 2-position, a substitution known to strongly reduce affinity for double-stranded DNA. Interactions of the modified drug on single-stranded DNAs of the defined sequence were investigated. Competition assays showed that 2-hydroxyactinomycin D has low affinity for two oligonucleotides that ...
متن کاملDna Dependence of Plastid Differentiation Inhibition by Actinomycin D
Euglena gracilis offers a particularly suitable system for investigating some of the fundamental processes involved in the provision, transmission, and translation of information required for the building of specific cytoplasmic structures. Cells grown in the dark do not have a defined chloroplast structure; they are etiolated cells (I). Light induces profound changes in the morphology and phys...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85741-1